Anti-p34 cdc2 antibody has been tested in ELISA and western blot and is suitable for immunoblotting, immunohistochemistry immunoprecipitation (as active kinase), and immunoblotting. HeLa cell lysate or human colon carcinoma is suggested as a positive con
Rocklands anti-cdc2 Cyclin Dependent Kinase was used to detect human p34cdc2 by western blot in untreated (Control) and drug treated lysates of MCF-7 cells. Lane 1-4 represents 3.1µM, 6.2µM, 12.5µM and 25.0µM genistein treatment of cells before lysates were prepared. Detection occurs using a 1:1,000 dilution. Although this antiserum detects non-specific bands at higher MW, a clear induction of signal is observed as the concentration of drug is increased. Personnel Communication, Xiao He Yang, University of Oklahoma Health Sciences Center.
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