This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 725-749 of Mouse STAT-4.
Konjugation:
Unconjugated
Alternative Synonym:
rabbit anti-STAT4 Antibody, Signal transducer and activator of transcription 4, Stat4
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Mouse
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:5,000 - 1:25,000, IP: Use 5 µL, WB: 1:5,000
Anwendungsbeschreibung:
This antibody has been tested for use in ELISA, western blotting and immunoprecipitation. This product was assayed by western blotting after immunoprecipitation of mouse STAT4 from a lysate containing over expressed protein. A 1:5,000 dilution shows reac
Western blot following immunoprecipitation of STAT4 using ROCKLAND Immunochemicals Rabbit-anti-STAT4 antibody. Lane 2 shows the detection of a single band corresponding to mouse STAT4. Molecular weight markers (not shown) confirm a MW of ~ 85 kDa. For IP use approximately 5 µl of antiserum. A 1:5,000 dilution of the antiserum was used for western blotting. Detection occurs using a 1:2,000 dilution of HRP Goat-a-Rabbit IgG (611-103-122) with visualization via ECL. Film exposure approximately 45. Other detection systems will yield similar results.
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