Liver Carboxylesterase-1 Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-100-401-B14
Artikelname: Liver Carboxylesterase-1 Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-100-401-B14
Hersteller Artikelnummer: 100-401-B14
Alternativnummer: ROC-100-401-B14
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: This whole rabbit serum was prepared by repeated immunizations with recombinant human Liver Carboxylesterase-1.
Konjugation: Unconjugated
Alternative Synonym: rabbit anti-Liver Carboxylesterase-1 Antibody, Liver carboxylesterase 1, Acyl coenzyme A:cholesterol acyltransferase, ACAT, Monocyte/macrophage serine esterase, HMSE, Serine esterase 1, Brain carboxylesterase hBr1, Triacylglycerol hydrolase, Egasyn, Retinyl ester hydrolase, REH
Klonalität: Polyclonal
Konzentration: 90 mg/mL by Refractometry
NCBI: 1066
UniProt: P23141
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:2,500 - 1:5,000
Anwendungsbeschreibung: This antibody has been tested for use in ELISA and western blotting and is specific for human CE1 (carboxylesterase). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 66 kDa in size corresponding CE1 by
Western blot using Rocklands anti-hCE1 shows detection of hCE1 (arrowhead) transfected into U373 MG (glioblastoma) whole cell lysate (lane 2) as a 66 kDa band. Purified hCE1 (lane 4) is similarly detected. The higher molecular weight band in lane 4 may represent a trimeric form of hCE1. No reactivity is seen in the untransfected control (lane 1). No cross reactivity occurs against the human intestinal form of CES1 (lane 3). The primary antibody was used at a 1:2,500 dilution to detect purified hCE1. Personal communication, Philip Potter and Jason Hatfield, St. Jude Childrens Research Hospital, Memphis, TN.