Anti-NMDA R2A Antibody has been tested by Western Blotting. Expect a band of approximately 180kDa in size corresponding to the NR2A subunit of the NMDA receptor in appropriate cell lysate or extract. A dilution of 1:1000 is recommended although specific
Western Blot of Anti-NMDA R2A (Rabbit) Antibody. Lane 1: rat hippocampal lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: NMDA R2A antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~180k, 180 kDa for NR2A subunit of the NMDA receptor. Other band(s): none.
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