BG37 Antibody was produced from whole rabbit serum which was prepared by repeated immunizations with the amino acid portion of human BG37 near the C-terminus of the chain .
Konjugation:
Unconjugated
Alternative Synonym:
TGR5, G-protein coupled bile acid receptor 1, G-protein coupled receptor GPCR19, hGPCR19, Membrane-type receptor for bile acids, M-BAR, hBG37, BG37
Anti-BG37 Antibody has been tested in WB. Expect a band approximately 37kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Rabbit anti-BG37 antibody. Lane 1: spleen lysate. Lane 2: human spleen lysate. Lane 3: mouse spleen lysate. Lane 4: rat spleen lysate. Load: 35 µg per lane. Primary antibody: BG37/TGR5 antibody at 1:1000 (hu) and 1:5000 (ms, rt) for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 35.2 kDa, 30 kDa for BG37/TGR5. Other band(s): 28kDa non-specific in mouse.
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