Eg5 was prepared from whole rabbit serum produced by repeated immunizations with a truncated Eg5 construct expressed in E. coli corresponding to human Eg5 protein.
Konjugation:
Unconjugated
Alternative Synonym:
rabbit anti-Eg5 antibody, Kinesin-like protein KIF11, Kinesin-5, KIF11, Kinesin-related motor protein Eg5, Thyroid receptor-interacting protein 5
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:10,000, IHC: user opimized, WB: 1:1000
Anwendungsbeschreibung:
Eg5 antibody has been tested by western blot. For western blots expect a band of approximately 72 kDa in size corresponding to truncated kinesin-1 protein. Specific conditions for reactivity should be optimized by the end user. This antibody is suitable
Western Blot of Rabbit Anti-Eg-5 Antibody. Lane 1: E.coli cell lysate expressing histidine tagged protein. Lane 2: Mouse brain lysate. Lane 3: Recombinant truncated Eg-5. Lane 4: MW Markers. Load: 35µg/lane for cell lysate, 50ng of recombinant protein. Primary antibody: Eg-5 antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody (p/n 611-103-122) at 1:40,000 for 60 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 120 kDa and 72 kDa for Eg5.
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