This product has been assayed against 1.0 ug of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code 611-1302 and (ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-su
The correlative workflow combining immuno-fluorescence microscopy and immuno-electron microscopy with NanoSIMS imaging using Anti-Carbonic Anhydrase II Antibody.The cryo section (ca. 100nm thick) was imaged after simultaneous immunolocalization of carbonic anhydrase with anti-Alexa-associated secondary antibodies (aandb) and beta-tubulin observed in TEM by 10nm gold particles associated to the secondary antibody (candd). The fluorescence microscope images (aandb, zoom ina) and the TEM micrographs (candd, zoom inc) exhibit identical areas of the same thin section.eandfare NanoSIMS images showing the13C and15N distributions in the interior of a dinoflagellate symbiont. The area imaged is indicated by a red square inc. Scale bars:aandc: 5µm,bandd: 500nm,eandf: 2µm. Fig 4. PMID: 32647198.
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