IL 8 antibody has been tested for use in ELISA and western blot. It recognizes IL-8 found in cells, supernatants and other body fluids. Reactivity in other immunoassays is unknown.
Western Blot of Rabbit anti-Human IL-8 Antibody. Lane 1: Recombinant Human IL-8. Load: 50 ng per lane. Primary antibody: Rabbit anti-Human IL-8 Antibody 1:1,000 overnight at 4C. Secondary antibody: HRP Goat-a-rabbit secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 11 kDa, 11 kDa for Human IL-8.
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