Anti-Hsp40 Antibody has been tested in WB, IP, and IF. Expect a band approximately ~40kDa protein corresponding to the molecular mass of Hsp40 on SDS PAGE immunoblots. Also detects recombinant Hsp40. Specific conditions for reactivity should be optimized
Western Blot of rabbit anti-Hsp40 antibody. Lane 1: HeLa cell lysates. Lane 2: molecular weight markers. Load: 35 µg per lane. Primary antibody: Hsp40 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 38 kDa for Hsp40. Other band(s): none.
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