Anti-Swine IgG antibody has been tested in western blot and is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Rabbit anti-Swine IgG (H&L) Antibody. Lane 1: Swine IgG (p/n 014-0102). Load: 100 ng per lane. Primary antibody: Swine IgG (H&L) Antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight(TM) 649 goat anti-rabbit (p/n 611-143-002) at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa.
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