Cholesterol Oxidase Antibody, Goat, Polyclonal

Produktübersicht

• Artikelname: Cholesterol Oxidase Antibody, Goat, Polyclonal
• Artikelnummer: ROC-200-101-213S
• Hersteller Artikelnummer: 200-101-213S
• Alternativnummer: ROC-200-101-213S
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Bacteria
• Immunogen: Cholesterol Oxidase [Streptomyces]
• Konjugation: Unconjugated
• Alternative Synonym: goat anti-Cholesterol Oxidase Antibody, choA antibody, choD antibody, choM antibody, choS antibody, COD antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/ml by UV absorbance at 280 nm
• NCBI: 12676
• UniProt: P12676
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Streptomyces
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Anwendungsbeschreibung: Anti-Cholesterol Oxidase Antibody has been tested by western blot. This product is suitable to be assayed against 1.0 ug of Cholesterol Oxidase [Microorganism] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit)

Bilder

Rockland Goat anti Cholesterol oxidase antibody (200-101-213 lot 6534) was used to detect purified cholesterol oxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified cholesterol oxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with a 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System.