Glucose Dehydrogenase Antibody, Goat, Polyclonal

Produktübersicht

• Artikelname: Glucose Dehydrogenase Antibody, Goat, Polyclonal
• Artikelnummer: ROC-200-101-214S
• Hersteller Artikelnummer: 200-101-214S
• Alternativnummer: ROC-200-101-214S
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Bacteria
• Immunogen: Glucose Dehydrogenase [Bacillus species]
• Konjugation: Unconjugated
• Alternative Synonym: goat anti-Glucose Dehydrogenase Antibody, gdh antibody, gdh2 antibody, gdh3 antibody, gdh4 antibody, gdhI antibody, GLCDHIII antibody, GLCDHIV antibody, Glucose 1 dehydrogenase 1 antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/ml by UV absorbance at 280 nm
• NCBI: 8985276
• UniProt: P07999
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Bacillus
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
• Anwendungsbeschreibung: Anti-Glucose Dehydrogenase Antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Glucose Dehydrogenase [Bacillus species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code

Bilder

Rockland Goat anti Glucose Dehydrogenase antibody (100-101-214 lot 6454) was used to detect purified Glucose Dehydrogenase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System