N-Acylmannosamine-1-Dehydrogenase Antibody, Goat, Polyclonal

Produktübersicht

• Artikelname: N-Acylmannosamine-1-Dehydrogenase Antibody, Goat, Polyclonal
• Artikelnummer: ROC-200-101-228-0100
• Hersteller Artikelnummer: 200-101-228-0100
• Alternativnummer: ROC-200-101-228-0100
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Bacteria
• Immunogen: N-Acyl Mannosamine-1-Dehydrogenase [Recombinant Sequence from Pseudomonas expressed in E.coli ]
• Konjugation: Unconjugated
• Alternative Synonym: goat anti-N-Acylmannosamine-1-Dehydrogenase Antibody, N-acylmannosamine 1-dehydrogenase, NAM-DH

Produkteigenschaften

• Klonalität: Polyclonal
• NCBI: 7614553
• UniProt: P22441
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Pseudomonas
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Anwendungsbeschreibung: N-ACYLMANNOSAMINE-1-DEHYDROGENASE Antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of N-Acylmannosamine-1-Dehydrogenase [Pseudomonas] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&

Bilder

Rockland Goat anti N-acylmanosamino-1-Dehydrogenase antibody (200-101-228 lot 8181) was used to detect purified N-acylmanosamino-1-Dehydrogenase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System.