Protein A Antibody Peroxidase Conjugated, HRP, Goat, Polyclonal

Produktübersicht

• Artikelname: Protein A Antibody Peroxidase Conjugated, HRP, Goat, Polyclonal
• Artikelnummer: ROC-200-103-077
• Hersteller Artikelnummer: 200-103-077
• Alternativnummer: ROC-200-103-077
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Immunogen: Protein A [Staphylococcus aureus]
• Konjugation: HRP
• Alternative Synonym: goat anti-Protein A Antibody, HRP Conjugated goat anti-Protein A Antibody, Protein A Peroxidase, Protein A HRP Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:2,000-1:20,000, IHC: 1:250, WB: 1:500-1:2,000
• Anwendungsbeschreibung: Anti-Protein A Peroxidase Conjugated has been tested by ELISA and western blot and is assayed against 1.0 ug of Protein A [Staphylococcus aureus] in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-1

Bilder

Western Blot of Goat anti-Protein A Antibody. Lane 1: Protein A Reduced. Lane 2: Protein A Reduced. Lane 3: Protein A Non-Reduced. Lane 4: Protein A Non-Reduced. Load: Lane 1 and 3 - 1.0 µg per lane, Lane 2 and 4 - 0.25 ug per lane. Primary antibody: Protein A antibody at 1:5,000 for overnight at 4C. Secondary antibody: DyLight(TM) 649 goat secondary antibody at 1:10,000 for 90 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~50 kDa for Protein A. Other band(s): Protein A splice variants and isoforms.