Invertase Antibody Peroxidase Conjugated, HRP, Goat, Polyclonal

Produktübersicht

• Artikelname: Invertase Antibody Peroxidase Conjugated, HRP, Goat, Polyclonal
• Artikelnummer: ROC-200-103-189S
• Hersteller Artikelnummer: 200-103-189S
• Alternativnummer: ROC-200-103-189S
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Yeast
• Immunogen: Invertase [Candida]
• Konjugation: HRP
• Alternative Synonym: goat anti-Invertase Antibody HRP Conjugated, Peroxidase Conjugated goat anti-Invertase Antibody, Beta fructofuranosidase 2 antibody, Inv1 antibody, Saccharase antibody, Sucrase antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 3640338
• UniProt: P10594
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Candida
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,500
• Anwendungsbeschreibung: Anti-Invertase has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Invertase [Candida] in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 m

Bilder

Rockland Goat anti Invertase antibody (100-101-189 lot 6736) was used to detect purified invertase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT) . Images were collected using the BioRad VersaDoc System