Alpha-2-Macroglobulin Antibody Peroxidase Conjugated, HRP, Goat, Polyclonal

Produktübersicht

• Artikelname: Alpha-2-Macroglobulin Antibody Peroxidase Conjugated, HRP, Goat, Polyclonal
• Artikelnummer: ROC-200-103-207-0100
• Hersteller Artikelnummer: 200-103-207-0100
• Alternativnummer: ROC-200-103-207-0100
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: DOT, WB
• Spezies Reaktivität: Human
• Immunogen: a2-Macroglobulin [Human Plasma]
• Konjugation: HRP
• Alternative Synonym: goat anti-Alpha-2-Macroglobulin Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Alpha-2-Macroglobulin Antibody, A2M antibody, Alpha 2 M antibody, Alpha 2M antibody, CPAMD5 antibody, DKFZp779B086 antibody, FWP007 antibody, S863 7 antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/ml by UV absorbance at 280 nm
• NCBI: 2
• UniProt: P01023
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:400 - 1:2,000, IHC: 1:50 - 1:250, WB: 1:100 - 1:500
• Anwendungsbeschreibung: Anti-Alpha 2-Macroglobulin has been tested by dot blot and western blot and is suitable to be assayed against 1.0 ug of alpha-Macroglobulin [Human Plasma] in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code

Bilder

Rockland Goat anti Alpha-2-Macroglobulin antibody (200-101-207 lot 20172) was used to detect Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti goat (605-743-125 lot 20834 1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System.