Anti-Maltose Phosphorylase has a working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
Rockland Goat anti Maltose Phosphorylase antibody (200-101-230 lot 7850) was used to detect Maltose Phosphorylase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System
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