Anti-Uricase Antibody Biotin Conjugated, Goat, Polyclonal

Produktübersicht

• Artikelname: Anti-Uricase Antibody Biotin Conjugated, Goat, Polyclonal
• Artikelnummer: ROC-200-106-092S
• Hersteller Artikelnummer: 200-106-092S
• Alternativnummer: ROC-200-106-092S
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Bacteria
• Immunogen: Uricase [Bacillus species]
• Konjugation: Biotin
• Alternative Synonym: goat anti-uricase antibody, biotin conjugated goat anti-uricase antibody, Urate oxidase antibody, Factor-independent urate hydroxylase

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 3202700
• UniProt: Q5WBJ3
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Bacillus
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:1,000 - 1:2,500, IP: 1:100, WB: 1:500 - 1:1,000
• Anwendungsbeschreibung: Anti-Uricase Antibody has been tested by ELISA and western blot. This product is suitable to be assayed against 1.0 ug of Uricase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline

Bilder

Western Blot of Goat anti-Uricase Antibody (Bacillus Species) Biotin Conjugated. Lane 1: Uricase (Bacillus). Load: 50 ng per lane. Primary antibody: Goat anti-Uricase Antibody (Bacillus Species) Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 39 kDa, 39 kDa for Uricase.

Rockland Goat anti Uricase antibody was used to detect purified Uricase under reducing and non-reducing conditions. Samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE and reduced samples of purified Uricase contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Rockland Goat anti Uricase (200-101-092 lot 6732 1:5K in MB-0070, ON 4 C). Primary antibody was detected with Rockland Dylight 649 conjugated Donkey anti Goat (605-743-125 1:10K 1.5 hr RT in MB-070 and imaged on the BioRad VersaDoc imaging system.