Sarcosine Oxidase Antibody Biotin Conjugated, Goat, Polyclonal

Produktübersicht

• Artikelname: Sarcosine Oxidase Antibody Biotin Conjugated, Goat, Polyclonal
• Artikelnummer: ROC-200-106-093S
• Hersteller Artikelnummer: 200-106-093S
• Alternativnummer: ROC-200-106-093S
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Bacteria
• Immunogen: Sarcosine Oxidase [Microbial]
• Konjugation: Biotin
• Alternative Synonym: goat anti-Sarcosine Oxidase Antibody, biotin Conjugated goat anti-Sarcosine Oxidase Antibody, Sarcosine oxidase subunit alpha, Sarcosine oxidase subunit

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 10586229
• UniProt: Q46337
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Microbial
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:1,000 - 1:2,500, IP: 1:100, WB: 1:500 - 1:1,000
• Anwendungsbeschreibung: Anti-Sarcosine Oxidase Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is suitable to be assayed against 1.0 ug of Sarcosine Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS

Bilder

Western Blot of Goat anti-Sarcosine Oxidase (Microbial) Antibody Biotin Conjugated. Lane 1: Sarcosine Oxidase (Microbial). Load: 50 ng per lane. Primary antibody: Goat anti-Sarcosine Oxidase (Microbial) Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Sarcosine Oxidase monomeric alpha subunit.

Rockland Goat anti Sarcosine Oxidase antibody was used to detect Sarcosine Oxidase. Samples of ~1 and 0.25 ug of purified Sarcosine Oxidase per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Rockland Goat anti Sarcosine Oxidase (200-101-093 lot 5951 1:5K in MB-0070, ON 4 C). Primary antibody was detected with Rockland Dylight 649 conjugated Donkey anti Goat (605-743-125 1:10K 1.5 hr RT in MB-070 and imaged on the BioRad VersaDoc imaging system.