Alpha-1-Anti-Trypsin Antibody Biotin Conjugated, Goat, Polyclonal

Produktübersicht

• Artikelname: Alpha-1-Anti-Trypsin Antibody Biotin Conjugated, Goat, Polyclonal
• Artikelnummer: ROC-200-106-147S
• Hersteller Artikelnummer: 200-106-147S
• Alternativnummer: ROC-200-106-147S
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: a1-Anti-Trypsin [Human Plasma]
• Konjugation: Biotin
• Alternative Synonym: goat anti-Alpha-1-Trypsin Antibody biotin Conjugation, biotin Conjugated goat anti-Alpha-1-Trypsin Antibody, A1AT antibody, AAT antibody, Alpha 1 antiproteinase antibody, Alpha 1 antitrypsin antibody, Alpha 1 protease inhibitor antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 5265
• UniProt: P01009
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:50,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Alpha-1-Anti-Trypsin has been tested by ELISA and western blot. This product is assayed against 1.0 ug of a-1-anti-Trypsin in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6

Bilder

Western Blot of goat anti-Alpha 1 anti trypsin antibody. Lane 1: molecular weight. Lane 2: Human transferrin (green), Alpha 1 anti trypsin (red), and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Load: 35 µg per lane. Primary antibody: Each primary antibody was diluted to 1:1000 for overnight at 4C. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight(TM) 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight(TM) 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight(TM) 488 Conjugated (blue)secondary antibody at 1:10,000 for ~ 1hr at 4 degrees. Block: Blocking Buffer for Fluorescent Western Blotting - MB-070 overnight at 4C

Western Blot of Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent Western blot. Lane 1: molecular weight. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 µg per lane. Primary antibody: Each primary antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: MB-070 at 30 min RT.

Western Blot of Goat anti Alpha-1 anti-Trypsin antibody. Lane 1: Alpha-1 anti-Trypsin under reducing conditions. Lane 2: none. Load: 1 µg per lane. Primary antibody: Alpha-1 anti-Trypsin antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: MB-070 overnight at 4C. Predicted/Observed size: 55kDa for alpha-1 Anti-Trypsin. Other band(s): none.