Invertase Antibody Biotin Conjugated, Goat, Polyclonal

Artikelnummer: ROC-200-106-189-0100
Artikelname: Invertase Antibody Biotin Conjugated, Goat, Polyclonal
Artikelnummer: ROC-200-106-189-0100
Hersteller Artikelnummer: 200-106-189-0100
Alternativnummer: ROC-200-106-189-0100
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Yeast
Immunogen: Invertase [Candida species]
Konjugation: Biotin
Alternative Synonym: goat anti-Invertase Antibody biotin Conjugated, Biotin Conjugated goat anti-Invertase Antibody, Beta fructofuranosidase 2 antibody, Inv1 antibody, Saccharase antibody, Sucrase antibody
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
NCBI: 3640338
UniProt: P10594
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Candida
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,500
Anwendungsbeschreibung: Anti-Invertase antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Invertase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid
Rockland Goat anti Invertase antibody (100-101-189 lot 6736) was used to detect purified invertase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT) . Images were collected using the BioRad VersaDoc System