Glycerol Kinase Antibody Biotin Conjugated, Goat, Polyclonal

Produktübersicht

• Artikelname: Glycerol Kinase Antibody Biotin Conjugated, Goat, Polyclonal
• Artikelnummer: ROC-200-106-211S
• Hersteller Artikelnummer: 200-106-211S
• Alternativnummer: ROC-200-106-211S
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Other
• Immunogen: Glycerol Kinase [Cellulomonas species]
• Konjugation: Biotin
• Alternative Synonym: goat anti-Glycerol Kinase Antibody biotin Conjugation, biotin Conjugated goat anti-Glycerol Kinase Antibody, ATP glycerol 3 phosphotransferase antibody, GK antibody, GK antibody, GK1 antibody, GKD antibody, Glycerokinase antibody, Glycerol kinase antibody, Glycerol kinase deficiency antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 6960048
• UniProt: D0VZG4
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Cellulomonas
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,500
• Anwendungsbeschreibung: Anti-Glycerol Kinase has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Glycerol Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfo

Bilder

Western Blot of Goat anti-Glycerol Kinase Antibody Biotin Conjugated. Lane 1: Glycerol Kinase. Load: 50 ng per lane. Primary antibody: Glycerol Kinase Antibody Biotin Conjugated at 1:1000 overnight at 4C. Secondary antibody: HRP Streptavidin (p/n S000-03) secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Glycerol Kinase.

Rockland Goat anti Glycerol Kinase antibody (200-101-211 lot 5912) was used to detect purified Glycerol Kinase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System.