Bilirubin Oxidase Antibody Biotin Conjugated, Goat, Polyclonal

Produktübersicht

• Artikelname: Bilirubin Oxidase Antibody Biotin Conjugated, Goat, Polyclonal
• Artikelnummer: ROC-200-106-212S
• Hersteller Artikelnummer: 200-106-212S
• Alternativnummer: ROC-200-106-212S
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Fungi
• Immunogen: Bilirubin Oxidase [Myrothecium verrucaria]
• Konjugation: Biotin
• Alternative Synonym: goat anti-Bilirubin Oxidase biotin conjugation, goat anti-Bilirubin Oxidase Antibody biotin Conjugated

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 12737
• UniProt: Q12737
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Myrothecium verrucaria
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:1,000 - 1:5,000, IP: 1:100, WB: 1:500 - 1:2,500
• Anwendungsbeschreibung: Anti-Bilirubin Oxidase has been tested by western blot and is suitable to be assayed against 1.0 ug of Bilirubin Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfon

Bilder

Rockland Goat anti Bilirubin Oxidase antibody (200-101-212 lot 6533) was used to detect Bilirubin Oxidase under reducing (R) conditions. A samples of ~1ug of purified protein contained 4% BME and was boiled for 5 minutes and run by SDS-PAGE on a 4-20% acrylamide gel. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT) and Dylight 649 conjugated Donkey anti goat (605-743-125 lot 20834 1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System

Western Blot of Goat anti-Bilirubin Oxidase (Myrothecium verrucaria) Antibody Biotin Conjugated. Lane 1: Bilirubin Oxidase (Myrothecium verrucaria). Load: 50 ng per lane. Primary antibody: Goat anti-Bilirubin Oxidase (Myrothecium verrucaria) Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin (p/n S000-03) 1:40,000 in MB-070 for 30 min at RT. Block: MB-070 for 30 minutes at RT. Predicted/Observed size: 64 kDa, 60 kDa for Bilirubin Oxidase. Other band(s): Bilirubin Oxidase splice variants and isoforms.