Pyruvate Kinase Antibody, Goat, Polyclonal

Artikelnummer: ROC-200-1178-0100
Artikelname: Pyruvate Kinase Antibody, Goat, Polyclonal
Artikelnummer: ROC-200-1178-0100
Hersteller Artikelnummer: 200-1178-0100
Alternativnummer: ROC-200-1178-0100
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Rabbit
Immunogen: Pyruvate Kinase (Rabbit Muscle)
Konjugation: Unconjugated
Alternative Synonym: goat anti-Pyruvate Kinase Antibody, Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody, PKM2
Klonalität: Polyclonal
Konzentration: 1.0 mg/ml by UV absorbance at 280 nm
NCBI: 100008676
UniProt: P11974
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Rabbit
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:4,000 - 1:20,000, IHC: 1:500 - 1:2,000, WB: 1:500 - 1:2,000
Anwendungsbeschreibung: This affinity purified antibody has been tested in western blot. This antibody is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Goat Anti-Pyruvate Kinase Antibody. Lane 1: CHO K1 Lysate 10µg. Lane 2: CHO S Lysate 10µg. Lane 3: Opal Pre-stained Molecular Weight Marker (p/n MB-210-0500). Primary Antibody: Goat Anti-Pyruvate Kinase at 1:50 overnight at 2-8C. Secondary Antibody: Donkey Anti-Goat IgG CY5 conjugate (p/n 605-710-125) at 1:10,000 for 30 mins at RT. Expect: ~57kDa.