This affinity purified antibody has been tested in western blot. This antibody is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Goat Anti-Pyruvate Kinase Antibody. Lane 1: CHO K1 Lysate 10µg. Lane 2: CHO S Lysate 10µg. Lane 3: Opal Pre-stained Molecular Weight Marker (p/n MB-210-0500). Primary Antibody: Goat Anti-Pyruvate Kinase at 1:50 overnight at 2-8C. Secondary Antibody: Donkey Anti-Goat IgG CY5 conjugate (p/n 605-710-125) at 1:10,000 for 30 mins at RT. Expect: ~57kDa.
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