Anti-Glucose Oxidase is useful in ELISA, western blot, and immunohistochemistry. Optimal titers should be reached by researchers.
Rockland Goat anti Glucose Oxidase antibody was used to detect Glucose Oxidase under reducing and non-reducing conditions. Reduced samples of purified Glucose Oxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1 and 0.25 µg of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Rockland Goat anti Glucose Oxidase antibody (200-1137 lot 165 1:5K in MB-070, ON 4 C). Primary antibody was detected with Rockland Dylight 649 conjugated Donkey anti Goat (605-743-125 lot 20834 1:10K 1.5 hr RT in MB-070 and imaged on the BioRad VersaDoc imaging system
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