Anti-Pepsin biotin antibody has been tested by ELISA and western. This product is suitable for Immunohistochemistry. Expect band at 41.2 kDa in appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Goat anti-Pepsin Antibody Biotin Conjugated. Lane 1: Pepsin. Load: 50 ng per lane. Primary antibody: Goat anti-Pepsin Antibody Biotin Conjugated 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin (p/n S000-03) at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 41 kDa, 41 kDa for Pepsin.
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