ELISA: 1:5,000, IHC: 1:50-1:100, IF Microscopy: User Optimized, IP: 1:50-1:100, WB: not recommended
Anwendungsbeschreibung:
Anti-Calcitonin Gene Related Peptide antibody has been tested by ELISA and ICC. This product is not recommended for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Suggested positive controls are dorsal ro
Immunocytochemistry of Anti-Calcitonin Gene Related Peptide (CGRP) (Mouse) Antibody. Tissue: cryopreserved DRG neurons. Fixation: grown on coverslips. Antigen retrieval: not required. Primary antibody: Calcitonin Gene Related Peptide antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: Calcitonin Gene Related Peptide is nuclear and occasionally cytoplasmic. Staining: CGRP (red). Green staining is PGP9.5. Yellow staining is the colocalization of CGRP and PGP9.5.
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