PUMA Antibody [2A8F6], IgG1, Mouse, Monoclonal

Produktübersicht

• Artikelname: PUMA Antibody [2A8F6], IgG1, Mouse, Monoclonal
• Artikelnummer: ROC-200-301-DV3
• Hersteller Artikelnummer: 200-301-DV3
• Alternativnummer: ROC-200-301-DV3
• Hersteller: Rockland Immunochemicals
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human, Rat
• Immunogen: Anti-PUMA antibody was produced In mice by repeated immunizations with a recombinant protein corresponding to amino acids 76 - 170 of human PUMA-alpha.
• Konjugation: Unconjugated
• Alternative Synonym: PUMA Antibody [2A8F6], JFY1, PUMA, JFY-1

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1 mg/mL by UV absorbance at 280 nm
• Klon-Bezeichnung: [2A8F6]
• Isotyp: IgG1
• NCBI: 27113
• UniProt: Q9BXH1
• Puffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:20,000 - 1:50,000, WB: 2.5-5 µg/mL
• Anwendungsbeschreibung: Anti-PUMA Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 21 kDa in Western Blots of specific cell lysates and tissues. Validated in hu

Bilder

Western Blot of Mouse anti-PUMA antibody. Lane A: K562 cell lysate at 2.5 µg/mL. Lane B: K562 cell lysate at 5 µg/mL. Primary antibody: PUMA antibody overnight at 4°C. Secondary antibody: Mouse HRP secondary antibody. Block: 5% BLOTTO. Predicted/Observed size: 20 kDa with 3 isoforms, 25 kDa for PUMA.