Anti-Syntaxin antibody is suitable for use in Western Blotting and IHC. Expect a band or approximately 36 kDa in size corresponding to syntaxin proteins in Western blot of rat brain lysate. Tested for use in immunohistochemistry on formalin fixed, vibrat
Western Blot of Mouse anti-Syntaxin antibody. Lane 1: rat cortex lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: Syntaxin antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 36 kDa for Syntaxin. Other band(s): none.
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