AKT3 Antibody, IgG1, Clone: [25F6.F6.D8], Mouse, Monoclonal

Produktübersicht

• Artikelname: AKT3 Antibody, IgG1, Clone: [25F6.F6.D8], Mouse, Monoclonal
• Artikelnummer: ROC-200-301-E75S
• Hersteller Artikelnummer: 200-301-E75S
• Alternativnummer: ROC-200-301-E75S
• Hersteller: Rockland Immunochemicals
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Anti-AKT3 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein.
• Konjugation: Unconjugated
• Alternative Synonym: Mouse anti-AKT3 antibody, AKT-3, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 3 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1.0 mg/ml by UV absorbance at 280 nm
• Klon-Bezeichnung: [25F6.F6.D8]
• Isotyp: IgG1
• NCBI: 10000
• UniProt: Q9Y243
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:2,000 - 1:10,000, Flow Cytometry: User Optimized, IHC: 20 µg/mL, WB: 1:500-1:2000
• Anwendungsbeschreibung: Anti-AKT3 Antibody is tested in ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts wit

Bilder

Immunohistochemistry of Mouse Anti-AKT3 antibody. Tissue: human prostate carcinoma. A) AKT-3 antibody produced using CELLine, B) AKT-3 antibody produced using roller bottle. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT-3 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 1 h at RT. Localization: AKT3 is nuclear and occasionally cytoplasmic. Staining: AKT3 as precipitated brown signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry of Mouse Monoclonal anti AKT3 Antibody in Mouse Embryonic Kidney. Tissue: Mouse Liver. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker. Primary antibody: anti-AKT3 at 2ug/ml for 1.5 hour room Temp. Secondary Ab: MOUSE ON MOUSE HRP POLYMER 45 RT.

Western Blot of Mouse anti-AKT3 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT3 antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT3. Other band(s): none.

Western Blot of Mouse Anti-AKT3 antibody. Lane 1: C2C12. Lane 2: MEF1. Lane 3: MEF2. Lane 4: A549. Lane 5: Calu-1. Lane 6: PC3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Load: 35 µg per lane. Primary antibody: AKT-3 antibody at 1:1000 for overnight at 4C. Secondary antibody: Anti mouse secondary antibody at 1:20,000 for 1 h at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 56 kDa for AKT3.

Western Blot of Mouse anti-AKT3 antibody. Lane 1: Control. Lane 2: Rapa. Lane 3: T50. Lane 4: T250. Lane 5: Control. Lane 6: Rapa. Lane 7: T50. Lane 8: T250. Lane 9: AKT3 null. Load: 35 µg per lane. Primary antibody: AKT-3 antibody at 1:1000 for overnight at 4C. Secondary antibody: Anti mouse secondary antibody at 1:20,000 for 1 h at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 56 kDa for AKT3.