Alpha B Crystallin Antibody, IgG1, Clone: [3A10.H4], Mouse, Monoclonal

Produktübersicht

• Artikelname: Alpha B Crystallin Antibody, IgG1, Clone: [3A10.H4], Mouse, Monoclonal
• Artikelnummer: ROC-200-301-E86
• Hersteller Artikelnummer: 200-301-E86
• Alternativnummer: ROC-200-301-E86
• Hersteller: Rockland Immunochemicals
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Bovine, Human
• Immunogen: Alpha B Crystallin Antibody was produced in mice by repeated immunizations with Native Alpha B Crystallin protein.
• Konjugation: Unconjugated
• Alternative Synonym: AACRYA, CRYA2, CRYAB, CTPP2, HspB5, NY Ren 27 antigen antibody, alpha crystallin B antibody, Heat shock protein beta-5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1mg/mL by UV absorbance at 280 nm
• Klon-Bezeichnung: [3A10.H4]
• Isotyp: IgG1
• NCBI: 1410
• UniProt: P02511
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:200, WB: 1:2000
• Anwendungsbeschreibung: Anti-Alpha B-crystallin Antibody has been tested in WB, IHC, and IF and is recommended for use in ELISA. Expect a band approximately ~20kDa (predicted mol. weight is ~21kDa) corresponding to alphaB-crystallin. Specific conditions for reactivity should be opt

Bilder

Western Blot of mouse Anti-Alpha B Crystallin Antibody. Lane 1: Rat Brain. Lane 2: Rat Heart. Lane 3: Rat Kidney. Lane 4: Rat liver. Lane 5: Rat lung. Lane 6: Rat Pancreas. Lane 7: Rat skeletal muscle. Lane 8: Rat Spleen. Lane 9: Rat testes. Lane 10:Rat thymus. Lane 11: C2C12 cell line. Lane 12: NIH 3T3 ctrl. Lane 13: PAM212 cell line. Load: 20ug per lane. Primary antibody: AlphaB-Crystallin 1:1000 for overnight at 4C. Secondary antibody: Goat anti-Mouse IgG HRP antibody at 1:40,000 for 45 min at RT. Block: 5% Blotto overnight at 4C. Predicted/Observed size: 19.9 kDa, 20 kDa for Alpha B Crystallin.