GRP78 Antibody, IgG1, Clone: [6H4-2G7], Mouse, Monoclonal

Produktübersicht

• Artikelname: GRP78 Antibody, IgG1, Clone: [6H4-2G7], Mouse, Monoclonal
• Artikelnummer: ROC-200-301-F37
• Hersteller Artikelnummer: 200-301-F37
• Alternativnummer: ROC-200-301-F37
• Hersteller: Rockland Immunochemicals
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: IF, WB
• Spezies Reaktivität: Bovine, Hamster, Human, Monkey, Mouse, Parasite, Rabbit, Rat, Xenopus
• Immunogen: Grp78 Antibody was produced in mice by repeated immunizations with a his-tagged human GRP78.
• Konjugation: Unconjugated
• Alternative Synonym: BiP, Grp78, HspA5, MIF2, immunoglobulin heavy chain binding protein, 78 kDa glucose-regulated protein, Heat shock 70 kDa protein 5

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1mg/mL by UV absorbance at 280 nm
• Klon-Bezeichnung: [6H4-2G7]
• Isotyp: IgG1
• NCBI: 3309
• UniProt: P11021
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:200, IF Microscopy: 1:100
• Anwendungsbeschreibung: Anti-GRP78 Antibody has been tested in WB and IF/ICC. Expect a band approximately ~78kDa corresponding to specific lysates. Specific conditions for reactivity should be optimized by the end user.

Bilder

Western Blot of mouse anti-Grp78 antibody. Lane 1: molecular weight. Lane 2: HeLa cell lysates. Load: 35 µg per lane. Primary antibody: Grp78 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 72.4 kDa, ~75 kDa for Grp78. Other band(s): none.