Anti-Vinculin antibody was prepared from whole mouse serum produced by repeated immunizations with a 23 amino acid peptide near the N-terminus of human Vinculin.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:500-1:10,000, WB: 0.5-1 µg/mL
Anwendungsbeschreibung:
Anti-Vinculin Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 124 kDa in Western Blots of specific cell lysates and tissues. Validated
Western Blot of Mouse anti-Vinculin antibody. Lane 1: 293 (p/n W09-000-365). Lane 2: A431 (p/n W09-000-361). Lane 3: A549 (p/n W09-001-372). Lane 4: HeLa (p/n W09-000-364). Lane 5: HepG2 (p/n W09-001-GJ5). Lane 6: K562 (p/n W09-001-GJ7). Lane 7: 3T3 (p/n W10-000-358). Lane 8: Raji (p/n W09-001-368). Lane 9: U937 (p/n W09-001-GL1). Lane 10: rat heart (p/n W12-000-T087). Lane 11: mouse lung tissue lysate. Lane 12: rat lung tissue lysate. Lane 13: mouse spleen (p/n W10-000-T029). Lane 14: rat spleen (p/n W12-000-T103). Lane 15: rabbit spleen tissue lysate. Lane 16: mouse brain (p/n W10-000-T004). Lane 17: rabbit brain tissue lysate. Lane 18: chicken spleen tissue lysate. Primary antibody: Anti-Vinculin at 1µg/mL O/N at 4°C. Secondary antibody: Mouse HRP antibody. Block: 5% BLOTTO. Predicted/Observed size: 117 kDa, 120 kDa for Vinculin.
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