0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:200, IF Microscopy: 1:100, WB: 1:1000
Anwendungsbeschreibung:
Anti-Piccolo Antibody is tested for use in WB, ICC/IF, and ELISA. Expect a band ~550kDa in specific lysate or tissue samples. Specific conditions for reactivity should be optimized by the end user.
Western Blot of mouse anti-Piccolo antibody. Lane 1: WT Mouse Brain Lysate. Lane 2: WT Rat Brain Lysate. Primary antibody: Piccolo antibody at 1:1000 for overnight at 4C. Secondary antibody: Goat anti-mouse IgG HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blotto overnight 4C. Predicted/Observed size: 547.5kDa/550kD.
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