BrdU Antibody, Clone: [29G6.E8], Mouse, Monoclonal

Produktübersicht

• Artikelname: BrdU Antibody, Clone: [29G6.E8], Mouse, Monoclonal
• Artikelnummer: ROC-200-301-H50
• Hersteller Artikelnummer: 200-301-H50
• Alternativnummer: ROC-200-301-H50
• Hersteller: Rockland Immunochemicals
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: DOT, IF
• Spezies Reaktivität: Other
• Immunogen: Anti-BrdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.
• Konjugation: Unconjugated
• Alternative Synonym: mouse anti-BrdU Antibody, Bromodeoxyuridine, 5-bromo-2-deoxyuridine, BrdU

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1.0 mg/ml by UV absorbance at 280 nm
• Klon-Bezeichnung: [29G6.E8]
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:2000 - 1:10000, Flow Cytometry: 1:50-1:100, IHC: 1:100-1:500, IF Microscopy: 1:500-1:3000, WB: 1:2000 - 1:5000
• Anwendungsbeschreibung: Anti-BrdU Antibody has been tested as suitable for immunofluorescence and immunoblot assays. Antibody may be suitable for additional immunoassays including flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by

Bilder

Immunofluorescence Microscopy of Mouse Anti-BrdU antibody. Tissue: OCT-embedded E10.5 mouse embryo. Localization: 20X, section through the developing limb bud. Fixation: 4% PFA. Antigen retrieval: not required. Primary antibody: BrdU antibody at 1:1000 in 0.4% PBS+Triton with 1% normal sheep serum overnight at 4C. Secondary antibody: Alexa Fluor 488 Anti-Mouse secondary antibody at 1:200 for 45 min at RT. Staining: Double labeled (green/blue) cells represent cells that were actively dividing.

Bromodeoxyuridine (BrdU) chemical structure representation.

Western blot of Anti-BrdU antibody. Lane 1: loading control. Lane 2: FdU. Lane 3: BrdU. Lane 4: IdU. Lane 5 CldU. Load: 20 µg per lane. Primary antibody: Anti-BrdU antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted: BrdU. Other band(s): cross reactive bands observed for other nucleoside analogs, IdU and CldU.

Immunofluorescence Microscopy of Mouse Anti-BrdU antibody. Tissue: OCT-embedded E10.5 mouse embryo. Localization: 20X, section through the developing hindbrain. Fixation: 4% PFA. Antigen retrieval: not required. Primary antibody: BrdU antibody at 1:1000 for overnight at 4C in 0.4% PBS+Triton with 1% normal sheep serum. Secondary antibody: Alexa Fluor 488 Anti-Mouse secondary antibody at 1:200 for 45 min at RT. Staining: Double labeled (green/blue) cells represent cells that were actively dividing.

Immunofluorescence Microscopy of Mouse Anti-BrdU antibody. Tissue: OCT-embedded E10.5 mouse embryo. Localization: 40X, section through the developing limb bud. Fixation: 4% PFA. Antigen retrieval: not required. Primary antibody: BrdU antibody at 1:500 in 0.4% PBS+Triton with 1% normal sheep serum overnight at 4C. Secondary antibody: Alexa Fluor 488 Anti-Mouse secondary antibody at 1:200 for 45 min at RT. Staining: Double labeled (green/blue) cells represent cells that were actively dividing.