IkB alpha Ser32 Ser36 Antibody was produced in mice prepared by repeated immunizations with a synthetic peptide containing phosphorylated serines at human IkBa 32 and 36.
Konjugation:
Unconjugated
Alternative Synonym:
IKBA, MAD3, NFKBI, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major histocompatibility complex enhancer-binding protein MAD3
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
IP: 1-2 µg/mL, WB: 1-2 µg/mL
Anwendungsbeschreibung:
Anti-IkBa Ser32 Ser36 Antibody is tested for use in IP, Peptide ELISA and WB. Expect a band approximately 35kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Mouse Anti-IkBa Ser32 Ser36 antibody. Jurkat cells treated with 100µg/mL pf ALLN for 30 min. Lane 1: Lysate without 1 nM TNF-a. Lane 2: Lysate with 1 nM TNF-a. Lane 3: Lysate without 1 nM TNF-a. Lane 4: Lysate with 1 nM TNF-a. Load: 30 µg per lane. Primary antibody: IkBa Ser32 Ser36 antibody at 4 µg/mL for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 35.6 kDa for IkBa Ser32 Ser36. Other band(s): both IkB alpha Phoshpo and non-phoshpo.
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