SARS-CoV-2 Nucleocapsid (N) Protein Antibody, IgG1, Clone: [7G9.C2], Mouse, Monoclonal

Artikelnummer: ROC-200-301-MS4
Artikelname: SARS-CoV-2 Nucleocapsid (N) Protein Antibody, IgG1, Clone: [7G9.C2], Mouse, Monoclonal
Artikelnummer: ROC-200-301-MS4
Hersteller Artikelnummer: 200-301-MS4
Alternativnummer: ROC-200-301-MS4
Hersteller: Rockland Immunochemicals
Wirt: Mouse
Kategorie: Antikörper
Applikation: ELISA
Spezies Reaktivität: Virus
Immunogen: This protein A purified antibody was produced by repeated immunizations with purified recombinant SARS-CoV-2 Nucleocapsid protein with C-terminal His-tag, derived from the transfected human HEK293 cells.
Konjugation: Unconjugated
Alternative Synonym: 2019-nCoV, COVID-19, N-protein antibody, SARS CoV2 antibody, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2, mouse anti-SARS CoV 2 Nucleocapsid Protein Antibody, sars-cov-2
Klonalität: Monoclonal
Konzentration: 1.18 mg/mL by UV absorbance at 280 nm
Klon-Bezeichnung: [7G9.C2]
Isotyp: IgG1
NCBI: 43740575
UniProt: P0DTC9
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: SARS
Antibody Type: Primary Antibody
Anwendungsbeschreibung: Anti-SARS-CoV-2 Nucleocapsid (N) Protein Monoclonal Antibody has been tested for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47 kDa in size corresponding to SARS-CoV-2 Nucleocapsid (N)
ELISA Results of Mouse Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody. Each well was coated in duplicate with 0.5 µg of SARS CoV-2 (N) Protein [Blue Line]. SARS CoV-2 N EC50: 5ng/mL. SARS CoV-2 S1 and SARS CoV-2 whole spike show no signal. The starting dilution of antibody was 0.55µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Goat Anti-Rabbit IgG HRP conjugated (p/n 611-1302) at 1:8000 and TMB substrate (p/n TMBE-1000).