AKT1 FITC Antibody, IgG2a, Clone: [5E5.F5.D7], Mouse, Monoclonal

Produktübersicht

• Artikelname: AKT1 FITC Antibody, IgG2a, Clone: [5E5.F5.D7], Mouse, Monoclonal
• Artikelnummer: ROC-200-302-J34
• Hersteller Artikelnummer: 200-302-J34
• Alternativnummer: ROC-200-302-J34
• Hersteller: Rockland Immunochemicals
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein followed by monoclonal development.
• Konjugation: FITC
• Alternative Synonym: mouse anti-AKT1 antibody FITC conjugation, fluorescein conjugated mouse anti-AKT-1 antibody, AKT-1, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 1 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Klon-Bezeichnung: [5E5.F5.D7]
• Isotyp: IgG2a
• NCBI: 207
• UniProt: P31749
• Puffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: User Optimized, Flow Cytometry: User Optimized, IHC: User Optimized, IF Microscopy: User Optimized, WB: User Optimized
• Anwendungsbeschreibung: Anti-AKT1 FITC Antibody has been tested by ELISA and western blot and is suitable for Flow Cytometry, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT1 protein by western blotting in the appropri

Bilder

Flow Cytometry of Mouse anti-AKT1 antibody. Cells: LNCap Cells. Stimulation: none. Primary antibody: Allophycocyanin AKT1 antibody at 1.0 µg/mL for 20 min at 4C.

Western Blot of Mouse anti-AKT1 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Lane 4: Molecular Weight Marker. Load: 25 ng per lane. Primary antibody: AKT1 antibody at 1:1000 for overnight at 4C. Secondary antibody: Mouse secondary antibody at 1:40,000 for 30 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 78 kDa for AKT1. Other band(s): none.

Western Blot of Mouse Anti-AKT1 antibody. Lane 1: Molecular Weight Marker. Lane 2: LnCap lysate (p/n W09-001-GJ9). Lane 3: Jurkat lysate (p/n W09-001-370). Lane 4: MDA-MB 468 lysate (p/n W09-001-GG9). Load: 5 µg per lane. Primary antibody: AKT1 antibody at 1:1000 for overnight at 4C. Secondary antibody: Mouse secondary antibody at 1:20,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 56 kDa for AKT1.

ELISA of Mouse Monoclonal anti-AKT1 antibody. Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well. Primary antibody: Mouse monoclonal anti-AKT1 antibody at 100 ng/well. Dilution series: 2-fold. Mid-point concentration: 3 ng/mL Mouse monoclonal anti-AKT1 antibody. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE-0100).

Western Blot of Mouse Anti-AKT1 Fluorescein Conjugated Antibody.Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: AKT1 Recombinant Protein (0.05µg). Lane 3: AKT2 Recombinant Protein (0.05µg). Lane 4: AKT3 Recombinant Protein (0.05µg). Lane 5: HEPG2 Whole Cell Lysate (20µg). Lane 6: C2C12 Whole Cell Lysate (20µg). Lane 7: A549 Whole Cell Lysate (20µg). Primary Antibody: Mouse Anti-AKT1 FITC at 1.0µg/mL overnight at 2-8C. Secondary Antibody: Rabbit Anti-Mouse HRP (p/n 610-403-C46) at 1:40,000 for 30mins at RT.Blocking: Fluorescent Buffer (p/n MB-073) for 1hr at RT.Predicted MW: ~56kDa. Exposure: 0.5 sec.