6X His Tag Antibody Dylight(TM) 488 Conjugated, IgG1, Clone: [33D10.D2.G8], DL488, Mouse, Monoclonal

Produktübersicht

• Artikelname: 6X His Tag Antibody Dylight(TM) 488 Conjugated, IgG1, Clone: [33D10.D2.G8], DL488, Mouse, Monoclonal
• Artikelnummer: ROC-200-341-382
• Hersteller Artikelnummer: 200-341-382
• Alternativnummer: ROC-200-341-382
• Hersteller: Rockland Immunochemicals
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Immunogen: This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
• Konjugation: DL488
• Alternative Synonym: mouse anti-6X His Tag DyLight(TM) 488 conjugated Antibody, DyLight(TM) 488 conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Klon-Bezeichnung: [33D10.D2.G8]
• Isotyp: IgG1
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: >1:20,000, IF Microscopy: >1:5,000, WB: >1:10,000-1:25,000
• Anwendungsbeschreibung: Anti-6X His epitope tag DyLight(TM) 488 has been tested by ELISA and western blot. The emission spectra for this DyLight(TM) conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Bilder

DyLight(TM) dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. DyLight(TM) dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods. This image shows anti-histone detection using a DyLight(TM) 488 conjugate (green). Anti-Tubulin was detected using a DyLight(TM) 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).

Properties of DyLight(TM) Fluorescent Dyes - Graph

Properties of DyLight(TM) Fluorescent Dyes.

Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.

ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins. Coating amount: 0.15ug per well. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE-1000).