AKT phospho T308 Dylight 549 Conjugated Antibody, IgG1, Clone: [18F3.H11], DL549, Mouse, Monoclonal

Produktübersicht

• Artikelname: AKT phospho T308 Dylight 549 Conjugated Antibody, IgG1, Clone: [18F3.H11], DL549, Mouse, Monoclonal
• Artikelnummer: ROC-200-342-269
• Hersteller Artikelnummer: 200-342-269
• Alternativnummer: ROC-200-342-269
• Hersteller: Rockland Immunochemicals
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Human, Monkey, Mouse, Rat
• Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein.
• Konjugation: DL549
• Alternative Synonym: mouse anti-AKT pT308 DyLight(TM)549 conjugated Antibody, DyLight(TM) 549 conjugated mouse anti-AKT pT308 Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Klon-Bezeichnung: [18F3.H11]
• Isotyp: IgG1
• NCBI: 207
• UniProt: P31749
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:4,000 - 1:20,000, FLISA: >1:20,000, Flow Cytometry: User Optimized, IHC: 20 µg/mL, IF Microscopy: >1:5,000, WB: >1:10,000
• Anwendungsbeschreibung: This monoclonal antibody is tested in western blotting. This antibody is suitable for immunofluorescence microscopy, IHC, FLISA, and flow cytometry. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blottin

Bilder

Properties of DyLight(TM) Fluorescent Dyes.

Immunohistochemistry of Mouse anti-AKT pT308 antibody. Tissue: human brain cerebellum tissue (40X). Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT pT308antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: staining of Purkinje neurons and cell processes in the cerebellum, cytosolic as well as occasionally nuclear. Staining: AKT pT308 as precipitated red signal with hematoxylin purple nuclear counterstain.

Western Blot of Mouse anti-AKT pT308 antibody DyLight 549 Conjugated. Lane 1: GST-AKT1 Unactive recombinant protein. Lane 2: GST-AKT1 Active recombinant protein. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: DyLight 549 conjugated Ms-a-AKT pT308 (p/n 200-342-269) was used at a 1:1,000 dilution for 1 h at RT. Block: 3% BSA (p/n BSA-30) in TBS for 30 min at RT. Predicted/Observed size: (indicated by arrowhead at ~79 kDa). Other band(s): None.

DyLight(TM) dyes can be used for two-color western blot detection with low background and high signal. Anti-tubulin was detected using a DyLight(TM) 549 conjugate. Anti-TNFa was detected using a DyLight(TM) 649 conjugate. The image was captured using the Typhoon(TM) 9410 Imaging System.

DyLight(TM) 549 Fluorescence absorption and emission spectra.