6X His Tag Antibody Dylight(TM) 680 Conjugated, IgG1, Clone: [33D10.D2.G8], DL680, Mouse, Monoclonal

Produktübersicht

• Artikelname: 6X His Tag Antibody Dylight(TM) 680 Conjugated, IgG1, Clone: [33D10.D2.G8], DL680, Mouse, Monoclonal
• Artikelnummer: ROC-200-344-382
• Hersteller Artikelnummer: 200-344-382
• Alternativnummer: ROC-200-344-382
• Hersteller: Rockland Immunochemicals
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Immunogen: This antibody was prepared by repeated immunizations of mice with a synthetic peptide corresponding to the 6X HIS epitope tag (H-H-H-H-H-H) conjugated to KLH using maleimide.
• Konjugation: DL680
• Alternative Synonym: mouse anti-6X His Tag DyLight(TM) 680 conjugated Antibody, DyLight(TM) 680 conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Klon-Bezeichnung: [33D10.D2.G8]
• Isotyp: IgG1
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: >1:20,000, IF Microscopy: >1:5,000, WB: >1:10,000
• Anwendungsbeschreibung: Anti-6X His Epitope tag DyLight(TM)680 has been tested by ELISA and Western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. This prod

Bilder

Properties of DyLight(TM) Fluorescent Dyes.

ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins. Coating amount: 0.15ug per well. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE-1000).

Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.

DyLight(TM) dyes can be used for two-color western blot detection with low background and high signal. Anti-tubulin was detected using a DyLight(TM) 680 conjugate. Anti-TNFa was detected using a DyLight(TM) 800 conjugate. The image was captured using the Odyssey Infrared Imaging System developed by LI-COR.

Properties of DyLight(TM) Conjugates.