6X His Tag Antibody Dylight(TM) 800 Conjugated, IgG1, Clone: [33D10.D2.G8], DL800, Mouse, Monoclonal

Produktübersicht

• Artikelname: 6X His Tag Antibody Dylight(TM) 800 Conjugated, IgG1, Clone: [33D10.D2.G8], DL800, Mouse, Monoclonal
• Artikelnummer: ROC-200-345-382
• Hersteller Artikelnummer: 200-345-382
• Alternativnummer: ROC-200-345-382
• Hersteller: Rockland Immunochemicals
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: DOT, ELISA, WB
• Immunogen: This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
• Konjugation: DL800
• Alternative Synonym: mouse anti-6X His Tag DyLight(TM) 800 conjugated Antibody, DyLight(TM) 800 conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Klon-Bezeichnung: [33D10.D2.G8]
• Isotyp: IgG1
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: >1:20,000, IF Microscopy: >1:5,000, WB: 1:10,000-1:25,000
• Anwendungsbeschreibung: Mouse Anti-6X HIS EPITOPE TAG DyLight(TM)800 Conjugated has been tested in ELISA, dot blot, and western blot and is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins

Bilder

Properties of DyLight(TM) Conjugates.

ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins. Coating amount: 0.15ug per well. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE-1000).

DyLight(TM) dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight(TM) 680 conjugate. Anti-TNFalpha was detected using a DyLight(TM) 800 conjugate. The image was captured using the Odyssey Infrared Imaging System developed by LI-COR.

Western Blot showing detection of 6x HIS Epitope Tag. Lane 1: Molecular Weight Marker. Lane 2: Recombinant 6xHIS-SUMO-GFP. Lane 3: Nag1. Blocking with 5% Blotto (p/n B501-0500) 30 min at 20C. Detection: Anti-6x His Epitope Tag (MOUSE) Monoclonal Antibody DyLight(TM) 800 Conjugated (p/n 200-345-382) secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged on the LiCor Odyssey imaging system.

Properties of DyLight(TM) Fluorescent Dyes.

Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.