Anti-NF-Y (A) antibody was produced by repeated immunizations with a synthetic NF-Y (A subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Konjugation:
Unconjugated
Alternative Synonym:
rabbit anti-NF-Y Antibody, Nf-Y-A subunit, CAAT box DNA-binding protein subunit A, Nuclear transcription factor Y subunit A
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:10,000, WB: 1:1,000
Anwendungsbeschreibung:
Anti-NF-Y antibody is tested for western blotting and suitable for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Western Blot of Rabbit Anti-NF-Y(A subunit) Antibody. Lane 1: CHO-7 cells in the absence (I) of cholesterol. Lane 2: CHO-7 cells in the presence (S) of cholesterol. Load: Equivalent aliquots of chromatin from each sample containing ~50 µg of total protein per lane. Primary antibody: anti-NF-YA antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody (p/n 611-103-122) at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: doublet of ~ 42 kDa for NF-Y A.
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