This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y701 in the mouse STAT1 protein.
Konjugation:
Unconjugated
Alternative Synonym:
rabbit anti-Stat1 pY701 Antibody, DKFZp686B04100 antibody, ISGF 3 antibody, Signal transducer and activator of transcription 1 91kDa antibody, Signal transducer and activator of transcription 1 alpha/beta antibody, Signal transducer and activator of transcription 1 antibody
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Mouse
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:25,000, WB: 1:500 - 1:2,000
Anwendungsbeschreibung:
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat1 protein by wes
Western blot using Rocklands Protein A purified anti-Stat1 pY701 antibody shows detection of phosphorylated Stat1 (indicated by arrowhead at ~91 kDa) in K562 cells after 30 min treatment with 1Ku of IFN-alpha (lane 2). No reactivity is seen for non-phosphorylated Stat1 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution at 4 C, overnight. Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.
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