CCL2 Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: CCL2 Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-200-401-B60
• Hersteller Artikelnummer: 200-401-B60
• Alternativnummer: ROC-200-401-B60
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Bovine
• Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 76 amino acids of the mature bovine CCL2 protein.
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-CCL2 antibody, CCL-2, CCL 2, Chemokine (C-C motif) ligand 2, monocyte chemotactic (or chemoattractant) protein-1 (MCP-1)

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 281043
• UniProt: A5PJF7
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Bovine
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:4,500, WB: 1 µg/ml
• Anwendungsbeschreibung: This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 8.8 kDa in size corresponding to bovine CCL2 protein by western bl

Bilder

Western blot using Rocklands protein-A purified anti-bovine CCL2 antibody shows detection of recombinant bovine CCL2 at 8.8kDa raised in yeast. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with Rockland Inc. Anti-bovine CCL2. After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody (611-743-127).