Eotaxin Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Eotaxin Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-200-401-BB0
• Hersteller Artikelnummer: 200-401-BB0
• Alternativnummer: ROC-200-401-BB0
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human
• Immunogen: Anti-Eotaxin antibody was raised in rabbits against a peptide corresponding to amino acids near the C-terminus of human eotaxin.
• Konjugation: Unconjugated
• Alternative Synonym: Eotaxin Antibody, SCYA11, SCYA11, Eotaxin, C-C motif chemokine 11

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL by UV absorbance at 280 nm
• NCBI: 6356
• UniProt: P51671
• Puffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000, IF Microscopy: 10 µg/mL, WB: 1 µg/mL
• Anwendungsbeschreibung: Anti-Eotaxin Antibody has been tested for use in ELISA, Western Blotting, Immunocytochemistry, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 11 kDa in Western Blots of speci

Bilder

Immunocytochemistry of Rabbit anti-Eotaxin antibody. Cell Type: 293 cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-Eotaxin antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Eotaxin is extracellular. Staining: Eotaxin as precipitated brown signal with hematoxylin purple nuclear counterstain.

Immunofluorescence Microscopy of Rabbit anti-Eotaxin antibody. Cell Type: 293 cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: anti-Eotaxin antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Eotaxin is extracellular. Staining: Eotaxin as red fluorescent signal with bis-benzimide (blue) nuclear counterstain.

Western Blot of Rabbit anti-Eotaxin antibody. Lane 1: HeLa cell lysate containing 10 ng of full recombinant eotaxin using anti-eotaxin antibody at 1 µg/mL. Lane 2: HeLa cell lysate containing 2.5 ng of full recombinant eotaxin using anti-eotaxin antibody at 1 µg/mL. Lane 3: HeLa cell lysate containing 1 ng of full recombinant eotaxin using anti-eotaxin antibody at 1 µg/mL. Load: 35 µg per lane. Primary antibody: anti-eotaxin antibody at 1:400 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 128 kDa, 128 kDa for Eotaxin. Other band(s): Eotaxin splice variants and isoforms.