0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Borrelia burgdorferi
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: >1:5,000, WB: 1:1,000
Anwendungsbeschreibung:
Anti-ErpN/OspE antibody has been tested in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at 17.1 kDa in size corresponding to ErpN/OspE by Western blotting in the appropriate cell lysate or
Western blot showing detection of 0.1 µg recombinant proteins in Western blot. Lane 1: Molecular weight markers. Lane 2: MBP-ErpN/OspE fusion protein (arrow, 59.5 kDa expected MW). Lane 3: fusion protein (MBP-tagged) plus cleaved fusion proteins (without MBP). Lane 4: MBP alone. The lower bands are probably breakdown products. The upper bands in lane 3 are fusion protein (top band), or breakdown products of the fusion protein (bands in middle of blot). Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc(TM) MP 4000 imaging system (Bio-Rad).
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