0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Lyophilized
Target-Kategorie:
Borrelia burgdorferi
Antibody Type:
Primary Antibody
Application Verdünnung:
WB: 1:1,000
Anwendungsbeschreibung:
This protein-A purified antibody has been tested for use in Western blotting and in ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 20.7 kDa in size corresponding to Borrelia burgdorferi OspC protein
Western blot showing detection of 0.1 µg of recombinant OspC protein. Lane 1: Molecular weight markers. Lane 2: MBP-OspC fusion protein (arrow, expected MW: 63.1 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc(TM) MP 4000 imaging system (Bio-Rad).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
