p39 Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: p39 Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-200-401-C17
• Hersteller Artikelnummer: 200-401-C17
• Alternativnummer: ROC-200-401-C17
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Bacteria
• Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi p39 protein.
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-p39 Antibody, Basic membrane protein A, Borrelia burgdorferi bmpA, immunodominant antigen P39, membrane lipoprotein BmpA

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 1195220
• UniProt: Q45010
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Borrelia burgdorferi
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: >1:5,000, WB: 1:1,000
• Anwendungsbeschreibung: This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 35.4 kDa in size corresponding to Borrelia burgdorferi p39 protein by

Bilder

Western blot showing detection of 0.1 µg of recombinant p39 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p39 fusion protein (expected MW: 77.8 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc(TM) MP 4000 imaging system (Bio-Rad).