NOD2 Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: NOD2 Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-200-401-DC8
• Hersteller Artikelnummer: 200-401-DC8
• Alternativnummer: ROC-200-401-DC8
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human
• Immunogen: Anti-Rabbit NOD2 polyclonal antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to 16 amino acids at the N-terminus of human NOD2.
• Konjugation: Unconjugated
• Alternative Synonym: NOD2 Antibody, CD, ACUG, BLAU, IBD1, NLRC2, NOD2B, CARD15, CLR16.3, PSORAS1, Nucleotide-binding oligomerization domain-containing protein 2, Caspase recruitment domain-containing protein 15

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL by UV absorbance at 280 nm
• NCBI: 64127
• UniProt: Q9HC29
• Puffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000-1:40,000, IHC: 5 µg/mL, IF Microscopy: 10 µg/mL, WB: 1 to 4 µg/mL
• Anwendungsbeschreibung: Anti-NOD2 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 115 kDa in Western Blots of specif

Bilder

Immunofluorescence Microscopy of NOD2 antibody. Tissue: Jurkat cels. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: NOD2 antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: NOD2 is cytoplasmic and is also localized in the cell membrane and the basolateral cell membrane. Staining: NOD2 as red fluorescent signal.

Western Blot of NOD2 antibody. Lane 1: Jurkat cell lysate with NOD2 at 1 µg/mL. Lane 2: Jurkat cell lysate with NOD2 at 2 µg/mL. Lane 3: Jurkat cell lysate with NOD2 at 4 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 115.3 kDa, 95 kDa for NOD2. Other band(s): NOD2 splice variants and isoforms.

Immunocytochemistry of NOD2 antibody. Cells: Jurkat cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: NOD2 antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: NOD2 is cytoplasmic and is localized in the cell membrane and basolateral membrane. Staining: NOD2 as precipitated brown signal with hematoxylin blue nuclear counterstain.